Fully automated purification of nucleic acids or proteins using QIAGEN spin kits on the QIAcube eliminates the need for tedious manual steps. Sample preparation on the QIAcube follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute) (see flowchart). No change in purification chemistry is required (see table "The QIAcube automates proven QIAGEN spin-column kits").

The QIAcube automates proven QIAGEN spin-column kits

 

Procedure	Starting material	QIAGEN kit
Purification of bisulfite converted DNA	Bisulfite conversion reaction	EpiTect Bisulfite Kit
Purification of plasmid DNA	E. coli cultures	QIAprep Spin Miniprep Kit
DNA cleanup	Amplification reactions	QIAquick PCR Purification Kit MinElute PCR Purification Kit
	Agarose gel slices	QIAquick Gel Extraction Kit MinElute Gel Extraction Kit
	Polyacrylamide gel slices	QIAquick Gel Extraction Kit
	Enzymatic reactions	MinElute Reaction Cleanup Kit QIAquick Nucleotide Removal Kit
Purification of genomic DNA	Whole blood, body fluids, or buccal swabs	QIAamp DNA Blood Mini Kit
	Tissues	QIAamp DNA Mini Kit
	Stool	QIAamp DNA Stool Mini Kit
	Forensic and human identity samples	QIAamp DNA Investigator Kit
	FFPE tissue sections	QIAamp DNA FFPE Tissue Kit
	Animal blood or cells, tissues, and rodent tails	DNeasy Blood & Tissue Kit
	Plant cells and tissues	DNeasy Plant Mini Kit
	Yeast (enzymatically lysed)	DNeasy Blood & Tissue Kit  QIAamp DNA Mini Kit
Purification of bacterial DNA	Biologcal fluids, swabs, or bacterial cultures	QIAamp DNA Mini Kit
	Gram-negative bacteria	DNeasy Blood & Tissue Kit
	Gram-positive bacteria (enzymatically lysed)	DNeasy Blood & Tissue Kit  QIAamp DNA Mini Kit
Purification of viral nucleic acids	Plasma, serum, or cell-free body fluids	QIAamp MinElute Virus Spin Kit
Purification of total RNA	Animal cells and easy-to-lyse tissues	RNeasy Plus Mini Kit RNeasy Mini Kit
	FFPE tissue sections	RNeasy FFPE Kit
	Fatty tissues	RNeasy Lipid Tissue Mini Kit
	Fibrous tissues	RNeasy Fibrous Tissue Mini Kit
	Aqueous phase of tissue or cell homogenates treated with chloroform	miRNeasy Mini Kit
	Plant cells and tissues	RNeasy Plant Mini Kit
	Small amounts of tissues or small numbers of cells	RNeasy Micro Kit
	Bacteria	RNeasy Protect Bacteria Mini Kit
Purification of cellular RNA	Blood (lysed leukocytes)	QIAamp RNA Blood Mini Kit
Purification of viral RNA	Cell-free body fluids	QIAamp Viral RNA Mini Kit
Purification of microRNA	Aqueous phase of tissue or cell homogenates	miRNeasy Mini Kit
RNA cleanup	RNA	RNeasy MinElute Cleanup Kit RNeasy Mini Kit RNeasy Micro Kit
Simultaneous purification of DNA and RNA	Animal tissues and cells	AllPrep DNA/RNA Mini Kit
Purification of 6xHis-tagged proteins	E. coli cultures or eukaryotic cells	Ni-NTA Spin Kit
Depletion of serum proteins	Serum or plasma	Qproteome Albumin/IgG Depletion Kit Qproteome Murine Albumin Depletion Kit

Efficient, standardized purification

The QIAcube enables purification of highly pure nucleic acids or proteins, and performance is comparable to the manual procedure. See data for purification of highly pure plasmid DNA, genomic DNA, viral nucleic acids, RNA, and proteins, plus DNA cleanup.

Continually expanding range of protocols

The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. The range of protocols available is continually expanding, and additional QIAGEN protocols can be downloaded free of charge.

Unparalleled ease of use

The QIAcube takes ease of use to a new level. No external PC is required, saving valuable laboratory space. An integrated touch screen simplifies protocol selection, and clear on-screen messages guide you through worktable setup. For increased ease of use and high process safety, labware and accessories fit onto the worktable only in the correct orientation. In addition, a fully automated load check helps to ensure that samples, reagents, and labware are correctly loaded.

IQ/OQ service and documentation

QIAGEN Qualification Services help you to qualify your system for correction installation (IQ) and operation (OQ) in your laboratory. We can perform the IQ/OQ onsite to save time and costs. IQ/OQ packages consist of professional documentation and the IQ/OQ is performed by Support Specialists to ensure compliance with regulatory demands. Find out how our expertise can help you to qualify your system and comply with regulatory demands.

Applications

The QIAcube is highly suitable for academic research laboratories as well as pharmaceutical, biotechnology, and biomedical research laboratories performing applications such as:

• Sequencing/sequencing analysis
• Gene expression analysis
• Genotyping
• Proteomics

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